Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing     

XiaoFeng Li  Chao Li  Qin Zhou  GuoXiong Chen  PengShan Zhao 《寒旱区科学》2020,12(3):180-188

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.  相似文献   

广西土壤有机质空间变异特征及其影响因素研究   总被引：2，自引：0，他引：2  

钟聪  李小洁  何园燕  邱微文  李杰  张新英  胡宝清 《地理科学》2020,40(3):478-485

基于广西第二次土壤普查的270个土壤剖面资料,结合1∶50万数字化土壤类型图、土地利用类型图和气象监测数据等资料,利用地统计学和逐步回归分析等方法对广西表层土壤有机质空间变异特征及其影响因素进行了探究。结果表明：广西表层土壤有机质平均含量为3.11±2.19%,变异系数为70.72%,空间分布呈北高南低的趋势。广西表层土壤有机质空间分布受到自然和人为因素的共同影响,土壤类型、成土母质、海拔、土地利用、气候和坡度6个环境因子对全区土壤有机质含量变异的综合解释能力为47.9%。其中,土壤类型是最重要的影响因素,能独立解释其变异的36.0%,海拔和成土母质分别能独立解释28.5%和15.8%。气温对广西土壤有机质空间分布的影响比降水量更加显著,从而造成了广西土壤有机质整体呈南低北高的趋势。同时,土壤有机质对气温的敏感性在一定程度上受到降雨量的制约。此外,研究区农业耕作管理等因素对土壤有机质的影响也不容忽视。  相似文献   

GDJ人卫目视观测数据采集和处理系统的研制     

何妙婵 《天文研究与技术》1996,(1)

本文介绍了ＧＤＪ跟踪打印经纬仪在人造卫星的观测中如何应用计算机对多圈卫星的目视观测资料进行实时采集、处理等工作过程的原理和方法。  相似文献   

Preliminary investigation of mercury in bone tissues of skua and penguin in Antarctica using AFS and Synchrotron Radiation X-ray Fluorescence （SR-XRF）   总被引：1，自引：0，他引：1  

谢周清程邦波  孙立广黄宇营 何伟 《极地研究(英文版)》2005,16(1):33-40

Mercury （Hg） was investigated in bone tissues of skua （ Catharacta maccormick） and penguin （Pygoscelis adeliae） collected in the maritime Antarctic using atomic fluorescence spectrometry （AFS） and synchrotron radiation X-ray fluorescence （SR-XRF） method. The total levels of mercury in bone tissues of penguin and skua are much lower than those in other organs （e. g. , kidney, liver）. The toxic effects of mercury in bone tissues of seabirds in polar region are not known. We have used SR- XRF method to map the distribution of trace levels of mercury in bones. The levels of mercury are found to be enriched somewhere near the periosteal surface and/or endosteal surface. The distribution of mercury shows strongly correlation with that of some essential elements and probably poses negative effect on the bone metabolism inferring from the relationship of mercury with the other elements. These studies represent a first step toward understanding the toxic effects of mercury on bone of polar animals by suggesting the possible microscopic investigation.  相似文献   

中国古生教授学会微体古生物学分会举行代表大会和学术年会     

李家骧 《南方国土资源》1996,(4)

中国古生教授学会微体古生物学分会举行代表大会和学术年会中国古竽物学会微体古物学分会第五次会员代表大分暨第六次不术年会于１９９６年１月２４日到３０日在福州市召开。来自全国地质、石油、煤炭的和产、科研和大学的１００多位代表参加了这次大会和学术研讨。大会共...  相似文献   

地壳对海洋潮汐的响应   总被引：2，自引：2，他引：0  

郑文衡  王乘  何振  牛安福 《大地测量与地球动力学》2002,22(2):41-46

应用三维动态有限元方法研究了中国北部地区的地壳对邻近的渤海与黄海海平面变化的响应。虽然此应力场过于微弱不足以引发地震，但发现应力集中的位置及应力场变化较大的位置恰好与某些现代地震的震中一致。这一结果表明研究地壳对广泛分布的载荷的响应对研究区域地震构造是有帮助的。  相似文献   

The Relation between the Amplitude and the Period of Solar Cycles   总被引：3，自引：0，他引：3  

Zhan-Le Du  Hua-Ning Wang  Xiang-Tao He National Astronomical Observatories  Chinese Academy of Sciences  Beijing  Department of Astronomy  Beijing Normal University  Beijing 《中国天文和天体物理学报》2006,6(4):489-494

The maximum amplitudes of solar activity cycles are found to be well anti-correlated (r = -0.72) with the newly defined solar cycle lengths three cycles before (at lag -3) in 13-month running mean sunspot numbers during the past 190 years. This result could be used for predicting the maximum sunspot numbers. The amplitudes of Cycles 24 and 25 are estimated to be 149.5±27.6 and 144.3±27.6, respectively.  相似文献   

Re-Identification of the ＇Enigmatic＇ X-ray Source 1RXS J114003.0＋124112     

Jiang-Hua Wu Tigran Movsessian Yang Chen Xiang-Tao He Xu Zhou Jun Ma 《中国天文和天体物理学报》2006,6(4):430-434

  相似文献   

SDSS J143030.22-001115.1： A Misclassified Narrow-line Seyfert 1 Galaxy with Flat X-ray Spectrum     

Wei-Hao Bian  Quan-Ling Cui  Li-Hua Chao Department of Physics  Institute of Theoretical Physics  Nanjing Normal University  Nanjing Key Laboratory for Particle Astrophysics  Institute of High Energy Physics  Chinese Academy of Sciences  Beijing 《中国天文和天体物理学报》2006,6(3):281-286

We used multi-component profiles to model the Hβ and [OⅢ]λλ4959,5007 lines of SDSS J143030.22-001115.1, a narrow-line Seyfert 1 galaxy (NLS1) in a sample of 150 NLS1 candidates selected from the Sloan Digital Sky Survey (SDSS), Early Data Release (EDR). After subtracting the Hβ contribution from narrow line regions (NLRs), we found that its full width half maximum (FWHM) of broad Hβ line is nearly 2900km s~(-1) , significantly larger than the customarily adopted criterion of 2000 km s~(-1) . With its weak Fe Ⅱ multiples, we believe that SDSS J143030.22-001115.1 should not be classified as a genuine NLS1. When we calculate the virial black hole masses of NLS1s, we should use the Hβ linewidth after subtracting the NLR component.  相似文献   

A multi‐level parallelized substructuring‐frontal solution for coupled thermo/hydro/mechanical problems in unsaturated soil     

H.R. Thomas  H.T. Yang  Y. He  P.J. Cleall 《国际地质力学数值与分析法杂志》2003,27(11):951-965

This paper proposes a multi‐level parallelized substructuring–frontal combined algorithm for the analysis of the problem of thermo/hydraulic/mechanical behaviour of unsaturated soil. Temperature, displacement, pore water pressure and pore air pressure are treated as the primary variables in a non‐linear analysis. Details are given firstly of the substructuring–frontal combined approach. The incorporation of the algorithm in a multi‐level parallel strategy is then discussed. The parallel processing can thus be carried out at different substructural levels. The method thus developed impacts, in a positive way, on both computer storage requirement and execution time. Copyright © 2003 John Wiley & Sons, Ltd.  相似文献